Marzena Cios
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Information: 2009-2010 Master's thesis "Isolation new bacterial strains and characterization of their metabolic potential" supervisor: dr. hab. eng. Marcin Lukaszewicz 2008 Bachelor "Production, natural role and potential applications of biosurfactants" Supervisor: Dr. Anna Krasowska |
About: During the thesis from soil samples of various origin Bacillus strains secreting extracellular proteases were isolated, and the cultures of strains were optimized for efficient production of proteases. Moreover, random UV induced mutagenesis was used for the selection of strains with increased proteases production. Additionally, production of the protease by B. subtilis 168 and B. subtilis SWV215 strains overexpressing AprE gene was evaluated.
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Biosurfactants
We identified chemical structures of newly isolated biosurfactants which are secreted by Arctic microorganisms. We are investigating their antibacterial, antifungal properties as well as proapoptotic activity. Biosurfactants can be used as an antiseptics.
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Biogas
We are investigating and optimizing the efficiency of metanogenesis in bioreactors. We are also testing various substrates (industrial waste) as materials for biogas production. Microorganisms which participate in this process are investigated as e.g. biofilm producers.
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Bacteriophages
Bacteriophages or phages are bacterial viruses. We isolated and are now investigating phages which kill Bacillus strains. Beside the basic research, the collection of phages can be used in industry as for example eliminators of infections.
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Antiseptics
Fungal infections are difficult to treat because of the small number of effective drugs. Candida albicans resistance depends on the activity of ABC transporters (MDR, CDR). We have investigated strains and methods for finding new antifungal compounds (synthetic and natural) e.g. probiotic yeast Saccharomyces boulardii.