Information:

2005 -2010 PhD thesis: "The influence of Saccharomyces boulardii on Candida albicans virulence factors".

Supervisor: Dr. hab inż. Marcin Łukaszewicz

2004-2005 Master's thesis: “N-oxides as antioxidants for targeted action”

Supervisor: Dr. Anna Krasowska

 2003 Bachelor:  “Adaptations of microorganisms to life in an environment with reduced oxygen content”

Supervisor: Dr. Anna Krasowska

Short communications, participation in conference and prizes

1.   I prize for the best PhD thesis in V edition of 2010 Hasco-Lek Foundation Competition.

2. 24th International Conference on Yeast Genetics and Molecular Biology, 19-24.07.2009 Menchester, Anglia. Dziadkowiec D, Murzyn A, Korczyńska M, Kuźlak S, Łukaszewicz M, Krasowska A. The antagonistic effect of active ingredients secreted by Saccharomyces cerevisiae and Saccharomyces boulardii on Candida albicans germ tube formation and adhesion.

3. Joint Meeting on Medicinal Chemistry. 24-27.06.2009 Budapeszt, Węgry, Murzyn A, Dziadkowiec D, Krasowska A, Characterisation of E. coli 17-2 and E. faecalis ATCC29212 secreted compounds active against adhesion of  pathogenic microorganisms.

4. XII International Congres of Bacteriology and Applied Microbiology. XII International Congress of Mycology. 05.08-09.08.2008 Istanbuł, Turcja, Murzyn A., Korczyńska M., Krasowska A., Dziadkowiec D. The antagonistic effect of active ingredients secreted by Saccharomyces cerevisiae on Candida albicans virulence factors.

5. XII International Congress of Bacteriology and Applied Microbiology. XII International Congress of Mycology. 05.08-09.08.2008 Istanbuł, Turcja, Murzyn A., Krasowska A., Van der Mei H. C, Busscher H. J., Dziadkowiec D. The effect of Escherichia coli and Enterococcus faecalis and their biosurfactants on Candida  albicans adhesion.

6. 41st Conference of Polish Biochemistry Community, 12.09. – 15.09.2006 Białystok, Murzyn A., Łukaszewicz M., Dziadkowiec D., Stefanowicz P., Szewczuk Z., Krasowska A. Determination of agents from Saccharomyces boulardii culture inhibiting filamentation of Candida albicans.

7. The 16th Congress of the Internetional Society for Human and Animal Mycology 25-29.06.2006, Paris, France. Krasowska A., Murzyn A., Dziadkowiec D. The inhibitory effect of Saccharomyces boulardii on Candida albicans virulence factors.

8. Winter School “applications of genomics and bioinformatics in animal biotechnology”, 1.02 -3.02. 2006, Jastrzębiec, Poland, Polinceusz A., Murzyn A., Krasowska A., Dziadkowiec D., Łukaszewicz M., Modifications of fatty acids biosynthesis to obtain new animal feed.

About:

Within the master thesis the degree of toxicity of various N-oxides was tested using NCCLS method on Saccharomyces cerevisiae strains. IC50 values ​​were determined for the tested compounds against the peroxyl radicals using chemiluminescent method. The specificity of N-oxides in the extinction of superoxide anion and peroxyl radicals generated by using paraquat and TBHP was determined. Preliminary studies on  the degree of incorporation of the N-oxides into liposomes bilayer were performed.

Within the PhD thesis the influence of S. boulardii on main virulence factors of C. albicans was tested. The ability to adhere of yeast cells to plastic and intestinal cell lines was inhibited in the presence of S. boulardii cells and the compounds secreted by this microorganism. The S. boulardii cells and extract contained compounds secreted by it, reduced also the C. albicans filament formation and subsequent biofilm production. Addition of S. boulardii extract caused significant reduction of IL-8 transcript levels in Caco-2 cells infected by C. albicans. The preparative thin layer chromatography and GC/MS and ESI-MS analysis of the active fraction showed the presence of short chain fatty acids (caproic acid, caprylic acid, capric acid) and 2-phenylethanol. The most active against C. albicans filament formation was the capric acid.